DNA Antenna Tile-Associated Deoxyribozyme Sensor with Improved Sensitivity.

ABSTRACT: Some natural enzymes increase the rate of diffusion-limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in a three- to fourfold increase in sensitivity and up to a ninefold improvement in the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion-limited enzymes and to improve sensitivity of enzyme-based biosensors.

SUBMITTER: Cox AJ 

PROVIDER: S-EPMC5156317 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature