Molecular analysis of idiopathic subglottic stenosis for Mycobacterium species.

ABSTRACT:

Objectives/hypothesis

Idiopathic subglottic stenosis (iSGS) is an unexplained obstruction involving the lower laryngeal and upper tracheal airway. Persistent mucosal inflammation is a hallmark of the disease. Epithelial microbiota dysbiosis is found in other chronic inflammatory mucosal diseases; however, the relationship between tracheal microbiota composition and iSGS is unknown. Given the critical role for host defense at mucosal barriers, we analyzed tissue specimens from iSGS patients for the presence of microbial pathogens.

Methods

Utilizing 30 human iSGS, 20 intubation-related tracheal stenosis (iLTS), and 20 healthy control specimens, we applied molecular, immunohistochemical, electron microscopic, immunologic, and Sanger-sequencing techniques.

Results

With un

SUBMITTER: Gelbard A 

PROVIDER: S-EPMC5156582 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature