Structural implications of Ca²⁺-dependent actin-bundling function of human EFhd2/Swiprosin-1.

ABSTRACT: EFhd2/Swiprosin-1 is a cytoskeletal Ca²⁺-binding protein implicated in Ca²⁺-dependent cell spreading and migration in epithelial cells. EFhd2 domain architecture includes an N-terminal disordered region, a PxxP motif, two EF-hands, a ligand mimic helix and a C-terminal coiled-coil domain. We reported previously that EFhd2 displays F-actin bundling activity in the presence of Ca²⁺ and this activity depends on the coiled-coil domain and direct interaction of the EFhd2 core region. However, the molecular mechanism for the regulation of F-actin binding and bundling by EFhd2 is unknown. Here, the Ca²⁺-bound crystal structure of the EFhd2 core region is presented and structures of mutants defective for Ca²⁺-binding are also described. These

SUBMITTER: Park KR 

PROVIDER: S-EPMC5156911 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature