Lyme disease spirochaete Borrelia burgdorferi does not require thiamin.

ABSTRACT: Thiamin pyrophosphate (ThDP), the active form of thiamin (vitamin B₁), is believed to be an essential cofactor for all living organisms^1,2. Here, we report the unprecedented result that thiamin is dispensable for the growth of the Lyme disease pathogen Borrelia burgdorferi (Bb)³. Bb lacks genes for thiamin biosynthesis and transport as well as known ThDP-dependent enzymes⁴, and we were unable to detect thiamin or its derivatives in Bb cells. We showed that eliminating thiamin in vitro and in vivo using BcmE, an enzyme that degrades thiamin, has no impact on Bb growth and survival during its enzootic infectious cycle. Finally, high-performance liquid chromatography analysis reveals that the level of thiamin and its derivatives in Ixodes scapularis

SUBMITTER: Zhang K 

PROVIDER: S-EPMC5157048 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature