Identification of Human Cathelicidin Peptide LL-37 as a Ligand for Macrophage Integrin α_Mβ₂ (Mac-1, CD11b/CD18) that Promotes Phagocytosis by Opsonizing Bacteria.

ABSTRACT: LL-37, a cationic antimicrobial peptide, has numerous immune-modulating effects. However, the identity of a receptor(s) mediating the responses in immune cells remains uncertain. We have recently demonstrated that LL-37 interacts with the α_MI-domain of integrin α_Mβ₂ (Mac-1), a major receptor on the surface of myeloid cells, and induces a migratory response in Mac-1-expressing monocyte/macrophages as well as activation of Mac-1 on neutrophils. Here, we show that LL-37 and its C-terminal derivative supported strong adhesion of various Mac-1-expressing cells, including HEK293 cells stably transfected with Mac-1, human U937 monocytic cells and murine IC-21 macrophages. The cell adhesion to LL-37 was partially inhibited by specific Mac-1 antagonists, including m

SUBMITTER: Lishko VK 

PROVIDER: S-EPMC5157691 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature