Analysis of Sequence Variation and Risk Association of Human Papillomavirus 52 Variants Circulating in Korea.

ABSTRACT:

Introduction

Human papillomavirus (HPV) 52 is a carcinogenic, high-risk genotype frequently detected in cervical cancer cases from East Asia, including Korea.

Materials and methods

Sequences of HPV52 detected in 91 cervical samples collected from women attending Seoul St. Mary's Hospital were analyzed. HPV52 genomic sequences were obtained by polymerase chain reaction (PCR)-based sequencing and analyzed using Seq-Scape software, and phylogenetic trees were constructed using MEGA6 software.

Results

Of the 91 cervical samples, 40 were normal, 22 were low-grade lesions, 21 were high-grade lesions and 7 were squamous cell carcinomas. Four HPV52 variant lineages (A, B, C and D) were identified. Lineage B was the most frequently detected lineage, followed by lineage C. By a

SUBMITTER: Choi YJ 

PROVIDER: S-EPMC5158036 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature