Kinetics of transcription initiation directed by multiple cis-regulatory elements on the glnAp2 promoter.

ABSTRACT: Transcription initiation is orchestrated by dynamic molecular interactions, with kinetic steps difficult to detect. Utilizing a hybrid method, we aim to unravel essential kinetic steps of transcriptional regulation on the glnAp2 promoter, whose regulatory region includes two enhancers (sites I and II) and three low-affinity sequences (sites III-V), to which the transcriptional activator NtrC binds. By structure reconstruction, we analyze all possible organization architectures of the transcription apparatus (TA). The main regulatory mode involves two NtrC hexamers: one at enhancer II transiently associates with site V such that the other at enhancer I can rapidly approach and catalyze the σ⁵⁴-RNA polymerase holoenzyme. We build a kinetic model characterizing essential steps of t

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5159524 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature