Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruginosa.

ABSTRACT: Bacteria respond to environmental stresses using a variety of signaling and gene expression pathways, with translational mechanisms being the least well understood. Here, we identified a tRNA methyltransferase in Pseudomonas aeruginosa PA14, trmJ, which confers resistance to oxidative stress. Analysis of tRNA from a trmJ mutant revealed that TrmJ catalyzes formation of Cm, Um, and, unexpectedly, Am. Defined in vitro analyses revealed that tRNA^Met(CAU) and tRNA^Trp(CCA) are substrates for Cm formation, tRNA^Gln(UUG), tRNA^Pro(UGG), tRNA^Pro(CGG) and tRNA^His(GUG) for Um, and tRNA^Pro(GGG) for Am. tRNA^Ser(UGA), previously observed as a TrmJ substrate in Escherichia coli, was not modified by PA14 TrmJ. Position

SUBMITTER: Jaroensuk J 

PROVIDER: S-EPMC5159551 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature