Alternative splicing of the OCC-1 gene generates three splice variants and a novel exonic microRNA, which regulate the Wnt signaling pathway.

ABSTRACT: The Wnt signaling pathway is hyperactivated in most colorectal cancers (CRC). Finding new regulators of this pathway represents the potential for cancer diagnosis or treatment. OCC-1 was initially reported as an up-regulated gene in colon carcinoma, without knowing its mechanism of action. Here, two novel transcript variants and an exonic microRNA that originated from the OCC-1 gene are reported, showing positive effects on Wnt activity. Up-regulation of the known OCC-1 variant (assigned as OCC-1A/B) was limited to CRC, and its overexpression increased survival of CRC-originated SW480 cells (Wnt⁺), while resulting in apoptosis of Wnt-suppressed SW480 cells or HeLa cells (Wnt^-) detected by PI staining. Immunocytochemistry showed that the OCC-1A/B-encoded peptide was lo

SUBMITTER: Najafi H 

PROVIDER: S-EPMC5159651 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature