Denatonium induces secretion of glucagon-like peptide-1 through activation of bitter taste receptor pathways.

ABSTRACT:

Aims/hypothesis

This study was designed to ascertain whether human enteroendocrine cells express bitter taste receptors, and whether activation of these receptors with bitter-tasting ligands induces secretion of glucagon-like peptide-1 (GLP-1) and peptide YY (PYY).

Methods

We used human enteroendocrine NCI-H716 cells, isolated duodenal segments from mice, and whole mice as our experimental systems for investigating stimuli and mechanisms underlying GLP-1- and PYY-stimulated release. We measured hormone levels by ELISA and determined bitter taste receptor expression by real-time quantitative PCR. We adopted a pharmacological approach using inhibitors and enhancers of downstream signalling pathways known to be involved in bitter taste transduction in taste bud cells to investi

SUBMITTER: Kim KS 

PROVIDER: S-EPMC5160131 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature