Identification and Analysis of NaHCO₃ Stress Responsive Genes in Wild Soybean (Glycine soja) Roots by RNA-seq.

ABSTRACT: Soil alkalinity is a major abiotic constraint to crop productivity and quality. Wild soybean (Glycine soja) is considered to be more stress-tolerant than cultivated soybean (G. max), and has considerable genetic variation for increasing alkalinity tolerance of soybean. In this study, we analyzed the transcriptome profile in the roots of an alkalinity tolerant wild soybean variety N24852 at 12 and 24 h after 90 mM NaHCO₃ stress by RNA-sequencing. Compared with the controls, a total of 449 differentially expressed genes (DEGs) were identified, including 95 and 140 up-regulated genes, and 108 and 135 down-regulated genes at 12 and 24 h after NaHCO₃ treatment, respectively. Quantitative RT-PCR analysis of 14 DEGs showed a high consistency with their expressi

SUBMITTER: Zhang J 

PROVIDER: S-EPMC5161042 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature