Antimicrobial peptide exposure selects for Staphylococcus aureus resistance to human defence peptides.

ABSTRACT:

Background

The clinical development of antimicrobial peptides (AMPs) is currently under evaluation to combat the rapid increase in MDR bacterial pathogens. However, many AMPs closely resemble components of the human innate immune system and the ramifications of prolonged bacterial exposure to AMPs are not fully understood.

Objectives

We show that in vitro serial passage of a clinical USA300 MRSA strain in a host-mimicking environment containing host-derived AMPs results in the selection of stable AMP resistance.

Methods

Serial passage experiments were conducted using steadily increasing concentrations of LL-37, PR-39 or wheat germ histones. WGS and proteomic analysis by MS were used to identify the molecular mechanism associated with increased tolerance of AMPs. AMP-r

SUBMITTER: Kubicek-Sutherland JZ 

PROVIDER: S-EPMC5161045 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature