A Model of Somatic Hypermutation Targeting in Mice Based on High-Throughput Ig Sequencing Data.

ABSTRACT: Analyses of somatic hypermutation (SHM) patterns in B cell Ig sequences have important basic science and clinical applications, but they are often confounded by the intrinsic biases of SHM targeting on specific DNA motifs (i.e., hot and cold spots). Modeling these biases has been hindered by the difficulty in identifying mutated Ig sequences in vivo in the absence of selection pressures, which skew the observed mutation patterns. To generate a large number of unselected mutations, we immunized B1-8 H chain transgenic mice with nitrophenyl to stimulate nitrophenyl-specific λ⁺ germinal center B cells and sequenced the unexpressed κ L chains using next-generation methods. Most of these κ sequences had out-of-frame junctions and were presumably uninfluenced by selection. Despite bei

SUBMITTER: Cui A 

PROVIDER: S-EPMC5161250 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature