A Whole Blood Assay for AR-V7 and AR^v567es in Patients with Prostate Cancer.

ABSTRACT:

Purpose

Most prostate cancer mortality can be attributed to metastatic castration resistant prostate cancer, an advanced stage that remains incurable despite recent advances. The AR (androgen receptor) signaling axis remains active in castration resistant prostate cancer. Recent studies suggest that expression of the AR-V (AR splice variant) AR-V7 may underlie resistance to abiraterone and enzalutamide. However, controversy exists over the optimal assay. Our objective was to develop a fast and sensitive assay for AR-Vs in patients.

Materials and methods

Two approaches were assessed in this study. The first approach was based on depletion of leukocytes and the second one used RNA purified directly from whole blood preserved in PAXgene® tubes. Transcript expression was analyze

SUBMITTER: Liu X 

PROVIDER: S-EPMC5161406 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature