Transcriptional Regulation of Connective Tissue Metabolism Genes in Women With Pelvic Organ Prolapse.

ABSTRACT:

Objective

The aim of this study was to compare differences in expressions and relationships between key genes involved in extracellular matrix metabolism and tissue cellularity in women with and without pelvic organ prolapse (POP).

Methods

A total of 80 biopsies (anterior cuff, posterior cuff, and/or leading edge) were obtained from 30 women: n = 10 premenopausal without POP (controls), n = 10 premenopausal with POP, and n = 10 postmenopausal with POP. Quantitative reverse-transcriptase polymerase chain reaction was used to assess gene expression of bone morphogenetic protein 1 (BMP1), collagen types I (COL1) and III (COL3), relaxin family peptide receptor 1 (RXFP1), matrix metallopeptidase 2, and TIMP metallopeptidase inhibitors 2 and 3. Hematoxylin and eosin staining was u

SUBMITTER: Borazjani A 

PROVIDER: S-EPMC5161600 | biostudies-literature | 2017 Jan/Feb

REPOSITORIES: biostudies-literature