Full and Partial Agonism of a Designed Enzyme Switch.

ABSTRACT: Chemical biology has long sought to build protein switches for use in molecular diagnostics, imaging, and synthetic biology. The overarching challenge for any type of engineered protein switch is the ability to respond in a selective and predictable manner that caters to the specific environments and time scales needed for the application at hand. We previously described a general method to design switchable proteins, called "chemical rescue of structure", that builds de novo allosteric control sites directly into a protein's functional domain. This approach entails first carving out a buried cavity in a protein via mutation, such that the protein's structure is disrupted and activity is lost. An exogenous ligand is subsequently added to substitute for the atoms that were removed by mutati

SUBMITTER: Budiardjo SJ 

PROVIDER: S-EPMC5161622 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature