Single-cell analysis of mixed-lineage states leading to a binary cell fate choice.

ABSTRACT: Delineating hierarchical cellular states, including rare intermediates and the networks of regulatory genes that orchestrate cell-type specification, are continuing challenges for developmental biology. Single-cell RNA sequencing is greatly accelerating such research, given its power to provide comprehensive descriptions of genomic states and their presumptive regulators. Haematopoietic multipotential progenitor cells, as well as bipotential intermediates, manifest mixed-lineage patterns of gene expression at a single-cell level. Such mixed-lineage states may reflect the molecular priming of different developmental potentials by co-expressed alternative-lineage determinants, namely transcription factors. Although a bistable gene regulatory network has been proposed to regulate the specific

SUBMITTER: Olsson A 

PROVIDER: S-EPMC5161694 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature