Structure-activity relationship of the exopolysaccharide from a psychrophilic bacterium: A strategy for cryoprotection.

ABSTRACT: Microrganisms from sea ice, glacial and subglacial environments are currently under investigation due to their relevant ecological functions in these habitats, and to their potential biotechnological applications. The cold-adapted Colwellia psychrerythraea 34H produces extracellular polysaccharides with cryoprotection activity. We here describe the purification and detailed molecular primary and secondary structure of the exopolysaccharide (EPS) secreted by C. psychrerythraea 34H cells grown at 4°C. The structure was determined by chemical analysis and NMR. The trisaccharide repeating unit of the EPS is constituted by a N-acetyl quinovosamine unit and two residues of galacturonic acid both decorated with alanine. In addition, the EPS was tested in vitro showing a significant inhibitory eff

SUBMITTER: Casillo A 

PROVIDER: S-EPMC5166977 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature