In vivo bioluminescence imaging reveals copper deficiency in a murine model of nonalcoholic fatty liver disease.

ABSTRACT: Copper is a required metal nutrient for life, but global or local alterations in its homeostasis are linked to diseases spanning genetic and metabolic disorders to cancer and neurodegeneration. Technologies that enable longitudinal in vivo monitoring of dynamic copper pools can help meet the need to study the complex interplay between copper status, health, and disease in the same living organism over time. Here, we present the synthesis, characterization, and in vivo imaging applications of Copper-Caged Luciferin-1 (CCL-1), a bioluminescent reporter for tissue-specific copper visualization in living animals. CCL-1 uses a selective copper(I)-dependent oxidative cleavage reaction to release d-luciferin for subsequent bioluminescent reaction with firefly luciferase. The probe can detect phys

SUBMITTER: Heffern MC 

PROVIDER: S-EPMC5167165 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature