Proteome Profile of Starch Granules Purified from Rice (Oryza sativa) Endosperm.

ABSTRACT: Starch is the most important food energy source in cereals. Many of the known enzymes involved in starch biosynthesis are partially or entirely granule-associated in the endosperm. Studying the proteome of rice starch granules is critical for us to further understand the mechanisms underlying starch biosynthesis and packaging of starch granules in rice amyloplasts, consequently for the improvement of rice grain quality. In this article, we developed a protocol to purify starch granules from mature rice endosperm and verified the quality of purified starch granules by microscopy observations, I2 staining, and Western blot analyses. In addition, we found the phenol extraction method was superior to Tris-HCl buffer extraction method with respect to the efficiency in recovery of starch granule

SUBMITTER: Xing S 

PROVIDER: S-EPMC5167393 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature