Continuous de novo generation of spatially segregated hepatitis C virus replication organelles revealed by pulse-chase imaging.

ABSTRACT:

Background & aims

Like all positive-sense RNA viruses, hepatitis C virus (HCV) induces host membrane alterations for its replication. In chronically infected cells, it is not known whether these viral replication organelles are being continually resupplied by newly synthesized viral proteins in situ, or whether they are generated de novo. Here we aimed to study temporal events in replication organelles formation and maturation.

Methods

Here we use pulse-chase labeling in combination with confocal microscopy, correlative light electron microscopy and biochemical methods to identify temporally distinct populations of replication organelles in living cells and study the formation, morphogenesis as well as compositional and functional changes of replication organelles over time.

SUBMITTER: Wang H 

PROVIDER: S-EPMC5167665 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature