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Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63.

ABSTRACT: Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.

SUBMITTER: Habe H

PROVIDER: S-EPMC516849 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature

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Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp. strain DBF63.

Habe Hiroshi H Chung Jin-Sung JS Kato Hiroyuki H Ayabe Yuko Y Kasuga Kano K Yoshida Takako T Nojiri Hideaki H Yamane Hisakazu H Omori Toshio T

Journal of bacteriology 20040901 17

Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded ...[more]

PMID: 15317800

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