Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR.

ABSTRACT:

Background

Plasmids are widely used for molecular cloning or production of proteins in laboratory and industrial settings. Constant modification has brought forth countless plasmid vectors whose characteristics in terms of average plasmid copy number (PCN) and stability are rarely known. The crucial factor determining the PCN is the replication system; most replication systems in use today belong to a small number of different classes and are available through repositories like the Standard European Vector Architecture (SEVA).

Results

In this study, the PCN was determined in a set of seven SEVA-based expression plasmids only differing in the replication system. The average PCN for all constructs was determined by Droplet Digital PCR and ranged between 2 and 40 per chromosome

SUBMITTER: Jahn M 

PROVIDER: S-EPMC5168713 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature