High resolution 3D imaging of living cells with sub-optical wavelength phonons.

ABSTRACT: Label-free imaging of living cells below the optical diffraction limit poses great challenges for optical microscopy. Biologically relevant structural information remains below the Rayleigh limit and beyond the reach of conventional microscopes. Super-resolution techniques are typically based on the non-linear and stochastic response of fluorescent labels which can be toxic and interfere with cell function. In this paper we present, for the first time, imaging of live cells using sub-optical wavelength phonons. The axial imaging resolution of our system is determined by the acoustic wavelength (λ_a = λ_probe/2n) and not on the NA of the optics allowing sub-optical wavelength acoustic sectioning of samples using the time of flight. The transverse resolution is currently

SUBMITTER: Perez-Cota F 

PROVIDER: S-EPMC5171858 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature