Follicular growth after xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: dynamics and molecular aspects.

ABSTRACT:

Purpose

To study the influence of xenotransplantation on follicular recruitment and growth in cryopreserved/thawed human ovarian tissue.

Method

Two 3-mm pieces of cryopreserved/thawed human ovarian tissue obtained from female cancer patients (n = 11) were xenotransplanted into a subcutaneous neck pouch of 6-week-old ovarectomized SCID mice (n = 33) for 4 (n = 18) and 12 (n = 15) weeks.

Result

Thirty-two out of 33 mice survived the entire observation periods. Graft recovery rate was 95.58 % (65 of 68 grafts). The percentages of primordial follicles after 4 weeks (P < 0.001) and 12 weeks (P = 0.009) of grafting were significantly lower in comparison to pregraft controls. The percentage of secondary follicle was significantly higher after 4 weeks of grafting (P = 0.018)

SUBMITTER: Ayuandari S 

PROVIDER: S-EPMC5171895 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature