Central Role of CD169⁺ Lymph Node Resident Macrophages in the Adjuvanticity of the QS-21 Component of AS01.

ABSTRACT: Saponins represent a promising class of vaccine adjuvant. Together with the TLR4-ligand MPL, QS-21 is part of the Adjuvant System AS01, a key component of the malaria and zoster candidate vaccines that display demonstrated clinical efficacy. However, the mechanism of action of QS-21 in this liposomal formulation is poorly understood. Upon intra-muscular immunisation, we observed that QS-21 rapidly accumulated in CD169⁺ resident macrophages of the draining lymph node where it elicited a local innate immune response. Depletion of these cells abrogated QS-21-mediated innate cell recruitment to the lymph node, dendritic cell (DC) phenotypic maturation as well as the adjuvant effect on T-cell and antibody responses to co-administered antigens. DCs rather than lymph node-resident macr

SUBMITTER: Detienne S 

PROVIDER: S-EPMC5172233 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature