Immunodominance in allergic T-cell reactivity to Japanese cedar in different geographic cohorts.

ABSTRACT:

Background

Japanese cedar (JC) pollen is a common trigger for allergic rhinitis in Japan. Pollen proteins targeted by IgE, including Cry j 1 and Cry j 2, and isoflavone reductase (IFR) have been identified.

Objective

To compare antigen-specific IgE titers and T-cell responses to JC pollen-derived extract and peptides in cohorts with high and low pollen exposure.

Methods

Peripheral blood mononuclear cells from JC pollen allergic or nonallergic patients who have lived in Japan for at least 1 year and JC pollen allergic patients who have never been to Japan were tested for T-cell responses against JC pollen extract and peptide pools derived from Cry j 1, Cry j 2, or IFR. T-cell reactivity was assessed by interleukin 5 and interferon γ production by ELISPOT.

Results

SUBMITTER: Oseroff C 

PROVIDER: S-EPMC5172395 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature