Cell Cycle Constraints and Environmental Control of Local DNA Hypomethylation in α-Proteobacteria.

ABSTRACT: Heritable DNA methylation imprints are ubiquitous and underlie genetic variability from bacteria to humans. In microbial genomes, DNA methylation has been implicated in gene transcription, DNA replication and repair, nucleoid segregation, transposition and virulence of pathogenic strains. Despite the importance of local (hypo)methylation at specific loci, how and when these patterns are established during the cell cycle remains poorly characterized. Taking advantage of the small genomes and the synchronizability of α-proteobacteria, we discovered that conserved determinants of the cell cycle transcriptional circuitry establish specific hypomethylation patterns in the cell cycle model system Caulobacter crescentus. We used genome-wide methyl-N6-adenine (m6A-) analyses by restriction-enzyme-

SUBMITTER: Ardissone S 

PROVIDER: S-EPMC5172544 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature