A General Method for Targeted Quantitative Cross-Linking Mass Spectrometry.

ABSTRACT: Chemical cross-linking mass spectrometry (XL-MS) provides protein structural information by identifying covalently linked proximal amino acid residues on protein surfaces. The information gained by this technique is complementary to other structural biology methods such as x-ray crystallography, NMR and cryo-electron microscopy[1]. The extension of traditional quantitative proteomics methods with chemical cross-linking can provide information on the structural dynamics of protein structures and protein complexes. The identification and quantitation of cross-linked peptides remains challenging for the general community, requiring specialized expertise ultimately limiting more widespread adoption of the technique. We describe a general method for targeted quantitative mass spectrometric anal

SUBMITTER: Chavez JD 

PROVIDER: S-EPMC5172568 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature