Frequency and type of inheritable mutations induced by γ rays in rice as revealed by whole genome sequencing.

ABSTRACT: Mutation breeding is based on the induction of genetic variations; hence knowledge of the frequency and type of induced mutations is of paramount importance for the design and implementation of a mutation breeding program. Although γ ray irradiation has been widely used since the 1960s in the breeding of about 200 economically important plant species, molecular elucidation of its genetic effects has so far been achieved largely by analysis of target genes or genomic regions. In the present study, the whole genomes of six γ-irradiated M₂ rice plants were sequenced; a total of 144-188 million high-quality (Q>20) reads were generated for each M₂ plant, resulting in genome coverage of >45 times for each plant. Single base substitution (SBS) and short insertion/deletion (I

SUBMITTER: Li S 

PROVIDER: S-EPMC5172596 | biostudies-literature | 2016 Dec.

REPOSITORIES: biostudies-literature