Genome-wide profiling of genetic variation in Agrobacterium-transformed rice plants.

ABSTRACT: Agrobacterium-mediated transformation has been widely used in producing transgenic plants, and was recently used to generate "transgene-clean" targeted genomic modifications coupled with the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas9) system. Although tremendous variation in morphological and agronomic traits, such as plant height, seed fertility, and grain size, was observed in transgenic plants, the underlying mechanisms are not yet well understood, and the types and frequency of genetic variation in transformed plants have not been fully disclosed. To reveal the genome-wide variation in transformed plants, we sequenced the genomes of five independent T₀ rice plants using next-generation sequencing (NGS) techniques. Bioinformatic

SUBMITTER: Li WX 

PROVIDER: S-EPMC5172603 | biostudies-literature | 2016 Dec.

REPOSITORIES: biostudies-literature