Characterization of Lgr5+ progenitor cell transcriptomes in the apical and basal turns of the mouse cochlea.

ABSTRACT: Lgr5+ supporting cells (SCs) are enriched hair cell (HC) progenitors in the cochlea, and several studies have shown a difference in the proliferation and HC regeneration ability of SCs between the apical and basal turns. However, the detailed differences between the transcriptomes of the apical and basal Lgr5+ SCs have not yet been investigated. We found that when isolated by FACS, Lgr5+ cells from the apex generated significantly more HCs and had significantly higher proliferation and mitotic HC regeneration ability compared to those from the base. Next, we used microarray analysis to determine the transcriptome expression profiles of Lgr5+ progenitors from the apex and the base. We first analyzed the genes that were enriched and differentially expressed in Lgr5+ progenitors from the apex

SUBMITTER: Waqas M 

PROVIDER: S-EPMC5173047 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature