Critical Role of LTB4/BLT1 in IL-23-Induced Synovial Inflammation and Osteoclastogenesis via NF-κB.

ABSTRACT: IL-23 activates the synthesis and production of leukotriene B₄ (LTB₄) in myeloid cells, which modulate inflammatory arthritis. In this study we investigated the role of LTB₄ and its receptor LTB₄R1 (BLT1) in synovial inflammation and osteoclast differentiation. Specifically, we used IL-23 in vivo gene transfer to induce arthritis in mice and showed that elevated serum LTB₄ and synovial expression of 5-lipoxygenase correlated with increased disease severity by histological evaluation and paw swelling compared with GFP gene transfer controls. To further investigate the effect of the LTB₄ pathway in bone loss, we performed osteoclast differentiation assays by stimulating with M-CSF and receptor activator of NF-κB ligand bone ma

SUBMITTER: Bouchareychas L 

PROVIDER: S-EPMC5173389 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature