Characterization of saliva microbiota's functional feature based on metagenomic sequencing.

ABSTRACT:

Objective

Saliva, a mixture of exocrinally secretive fluids, amounts to ~1.5 L daily and harbors numerous microbial inhabitants. However, except the organismal structure of saliva microbiota, the functional profile of saliva microbiota remain elusive.

Methods

Here we used metagenomic sequencing to experimentally reconstruct the global genomic profile of saliva by sequencing total saliva DNA from two healthy and two caries-active (DMFT ≧ 6) adults.

Results

We found that saliva microbiota, representing 30-60% of total saliva DNA in our samples, might carry functional signatures that were site-specific and caries-state-specific. Among microbiota from different hosts, a prominent functional core, but not an organismal core, was identified. Each microbiota exhibited functi

SUBMITTER: Yang F 

PROVIDER: S-EPMC5174016 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature