Identification of a Protein Network Driving Neuritogenesis of MGE-Derived GABAergic Interneurons.

ABSTRACT: Interneurons are essential modulators of brain activity and their abnormal maturation may lead to neural and intellectual disabilities. Here we show that cultures derived from murine medial ganglionic eminences (MGEs) produce virtually pure, polarized γ-aminobutyric acid (GABA)-ergic interneurons that can form morphologically identifiable inhibitory synapses. We show that Rac GTPases and a protein complex including the GIT family scaffold proteins are expressed during maturation in vitro, and are required for the normal development of neurites. GIT1 promotes neurite extension in a conformation-dependent manner, while affecting its interaction with specific partners reduces neurite branching. Proteins of the GIT network are concentrated at growth cones, and interaction mutants may af

SUBMITTER: Franchi SA 

PROVIDER: S-EPMC5174131 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature