Unraveling the genetic basis of xylose consumption in engineered Saccharomyces cerevisiae strains.

ABSTRACT: The development of biocatalysts capable of fermenting xylose, a five-carbon sugar abundant in lignocellulosic biomass, is a key step to achieve a viable production of second-generation ethanol. In this work, a robust industrial strain of Saccharomyces cerevisiae was modified by the addition of essential genes for pentose metabolism. Subsequently, taken through cycles of adaptive evolution with selection for optimal xylose utilization, strains could efficiently convert xylose to ethanol with a yield of about 0.46 g ethanol/g xylose. Though evolved independently, two strains carried shared mutations: amplification of the xylose isomerase gene and inactivation of ISU1, a gene encoding a scaffold protein involved in the assembly of iron-sulfur clusters. In addition, one of evolved strains carr

SUBMITTER: Dos Santos LV 

PROVIDER: S-EPMC5175268 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature