Release of periplasmic proteins of Brucella suis upon acidic shock involves the outer membrane protein Omp25.

ABSTRACT: The survival and replication of Brucella in macrophages is initially triggered by a low intraphagosomal pH. In order to identify proteins released by Brucella during this early acidification step, we analyzed Brucella suis conditioned medium at various pH levels. No significant proteins were released at pH 4.0 in minimal medium or citrate buffer, whereas in acetate buffer, B. suis released a substantial amount of soluble proteins. Comparison of 13 N-terminal amino acid sequences determined by Edman degradation with their corresponding genomic sequences revealed that all of these proteins possessed a signal peptide indicative of their periplasmic location. Ten proteins are putative substrate binding proteins, including a homologue of the nopaline binding protein of Agrobacterium tumefaciens

SUBMITTER: Boigegrain RA 

PROVIDER: S-EPMC517528 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature