Mutation analysis of NANOS3 in Brazilian women with primary ovarian failure.

ABSTRACT:

Objectives:

Primary ovarian failure is a rare disorder, and approximately 90% of cases are of unknown etiology. The aim of this study was to search for mutations in NANOS3, a gene that was recently related to the etiology of primary ovarian failure, in a group of Brazilian women.

Methods:

We screened for NANOS3 DNA variants in 30 consecutive women who were previously diagnosed with primary ovarian failure, of unknown etiology and compared the results with those from 185 women with normal fertility. The NANOS3 gene was amplified by polymerase chain reaction using pairs of specific primers and then sequenced. The resulting sequences were compared with control sequences available in the National Center for Biotechnology and Information database.

Results:

No mutations in

SUBMITTER: Sousa BL 

PROVIDER: S-EPMC5175295 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature