NanoStringDiff: a novel statistical method for differential expression analysis based on NanoString nCounter data.

ABSTRACT: The advanced medium-throughput NanoString nCounter technology has been increasingly used for mRNA or miRNA differential expression (DE) studies due to its advantages including direct measurement of molecule expression levels without amplification, digital readout and superior applicability to formalin fixed paraffin embedded samples. However, the analysis of nCounter data is hampered because most methods developed are based on t-tests, which do not fit the count data generated by the NanoString nCounter system. Furthermore, data normalization procedures of current methods are either not suitable for counts or not specific for NanoString nCounter data. We develop a novel DE detection method based on NanoString nCounter data. The method, named NanoStringDiff, considers a generalized linear m

SUBMITTER: Wang H 

PROVIDER: S-EPMC5175344 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature