Evolution of Arabidopsis protection of telomeres 1 alters nucleic acid recognition and telomerase regulation.

ABSTRACT: Protection of telomeres (POT1) binds chromosome ends, recognizing single-strand telomeric DNA via two oligonucleotide/oligosaccharide binding folds (OB-folds). The Arabidopsis thaliana POT1a and POT1b paralogs are atypical: they do not exhibit telomeric DNA binding, and they have opposing roles in regulating telomerase activity. AtPOT1a stimulates repeat addition processivity of the canonical telomerase enzyme, while AtPOT1b interacts with a regulatory lncRNA that represses telomerase activity. Here, we show that OB1 of POT1a, but not POT1b, has an intrinsic affinity for telomeric DNA. DNA binding was dependent upon a highly conserved Phe residue (F65) that in human POT1 directly contacts telomeric DNA. F65A mutation of POT1a_OB1 abolished DNA binding and diminished telomerase re

SUBMITTER: Arora A 

PROVIDER: S-EPMC5175356 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature