In vitro Degradation of Antimicrobials during Use of Broth Microdilution Method Can Increase the Measured Minimal Inhibitory and Minimal Bactericidal Concentrations.

ABSTRACT: The antibacterial activity of some antimicrobials may be under-estimated during in vitro microbiological susceptibility tests, due to their instability under such conditions. The in vitro degradation of seven widely used antimicrobials (amoxicillin, cephalexin monohydrate, cefotaxime sodium salt, ciprofloxacin, erythromycin hydrate, clarithromycin, and doxycycline hyclate) and its effect on MIC and MBC determinations was studied using the broth microdilution method, considered as the gold standard for MIC determinations. In vitro concentrations of antimicrobials, over a 24 h incubation period in the medium tested without bacteria, decreased from 33% for ciprofloxacin to 69% for clarithromycin. For cephalexin, cefotaxime, clarithromycin, and doxycycline which were the m

SUBMITTER: Lallemand EA 

PROVIDER: S-EPMC5175475 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature