Development and characterization of an in vivo central venous catheter Candida albicans biofilm model.

ABSTRACT: Biofilms represent a niche for microorganisms where they are protected from both the host immune system and antimicrobial therapies. Biofilm growth serves as an increasing source of clinical infections. Candida infections are difficult to manage due to their persistent nature and associated drug resistance. Observations made in biofilm research have generally been limited to in vitro models. Using a rat central venous catheter model, we characterized in vivo Candida albicans biofilm development. Time-course quantitative culture demonstrated a progressive increase in the burden of viable cells for the first 24 h of development. Fluorescence and scanning electron microscopy revealed a bilayered architecture. Adjacent to the catheter surface, yeast cells were densely embedded in an extracellu

SUBMITTER: Andes D 

PROVIDER: S-EPMC517581 | biostudies-literature | 2004 Oct

REPOSITORIES: biostudies-literature