Splice variant insertions in the C-terminus impairs YAP's transactivation domain.

ABSTRACT: The yes-associated protein (YAP) is a key effector of the mammalian Hippo signaling pathway. YAP has eight known alternately spliced isoforms and these are widely expressed across multiple tissues. Variable effects have been ascribed to different YAP isoforms by inducing their expression in cells, but whether these differences are due to variability in the transcriptional potency of individual YAP isoforms has not been addressed. Indeed a systematic comparison of the transcriptional potencies of YAP isoforms has not been done. To address this, using overexpression and transcriptional reporter analyses we investigated the transcriptional activities of several human YAP isoforms and determined the effects of the splice variant insertions within the transactivation domain on its transcription

SUBMITTER: Finch-Edmondson ML 

PROVIDER: S-EPMC5176130 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature