Phylodynamic and Phylogeographic Profiles of Subtype B HIV-1 Epidemics in South Spain.

ABSTRACT:

Background

Since 1982, HIV-1 epidemics have evolved to different scenarios in terms of transmission routes, subtype distribution and characteristics of transmission clusters. We investigated the evolutionary history of HIV-1 subtype B in south Spain.

Patients & methods

We studied all newly diagnosed HIV-1 subtype B patients in East Andalusia during the 2005-2012 period. For the analysis, we used the reverse transcriptase and protease sequences from baseline resistance, and the Trugene® HIV Genotyping kit (Siemens, Barcelona, Spain). Subtyping was done with REGA v3.0. The maximum likelihood trees constructed with RAxML were used to study HIV-1 clustering. Phylogeographic and phylodynamic profiles were studied by Bayesian inference methods with BEAST v1.7.5 and SPREAD v1.0.6.<

SUBMITTER: Perez-Parra S 

PROVIDER: S-EPMC5176287 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature