Metformin Improves Ileal Epithelial Barrier Function in Interleukin-10 Deficient Mice.

ABSTRACT:

Background and aims

The impairment of intestinal epithelial barrier is the main etiologic factor of inflammatory bowel disease. The proper intestinal epithelial proliferation and differentiation is crucial for maintaining intestinal integrity. Metformin is a common anti-diabetic drug. The objective is to evaluate the protective effects of metformin on ileal epithelial barrier integrity using interleukin-10 deficient (IL10KO) mice.

Methods

Wild-type and IL10KO mice were fed with/without metformin for 6 weeks and then ileum was collected for analyses. The mediatory role of AMP-activated protein kinase (AMPK) was further examined by gain and loss of function study in vitro.

Results

Compared to wild-type mice, IL10KO mice had increased proliferation, reduced goblet cell a

SUBMITTER: Xue Y 

PROVIDER: S-EPMC5176295 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature