A robust, low- to medium-throughput prnp genotyping system in sheep.

ABSTRACT:

Background

In many countries breeding programs for resistance to scrapie in sheep are established. Therefore, the demand on genotyping capacities of the polymorphisms of the prion protein gene (prnp) relevant to presently known disease associations and EU regulations is steadily increasing. Most published typing methods are not well suited for routine typing of large sample numbers in smaller service laboratories for different reasons: they require partly manual data processing, sophisticated and sensitive protocols, high efforts regarding time and manpower, multiple step reactions or substantial hardware investments. To overcome these drawbacks, we developed a prnp typing method that is based on a 'multiplex amplification refractory mutation system' (ARMS) reaction.

Methods

SUBMITTER: Buitkamp J 

PROVIDER: S-EPMC517712 | biostudies-literature | 2004 Sep

REPOSITORIES: biostudies-literature