A chemical synthesis of a multiply ¹³ C-labeled hexasaccharide: a high-mannose N-glycan fragment.

ABSTRACT: As covalent modifiers of proteins, high-mannose N-glycans are important in maintaining protein structure and function in vivo. The conformations of these glycans can be studied by nuclear magnetic resonance spectroscopy using spin-spin couplings (J-couplings; scalar couplings) and other nuclear magnetic resonance parameters that are sensitive to the geometries of their constituent glycosidic linkages and other mobile elements in their structures. These analyses often require ¹³ C-labeling at specific carbon atoms, especially when measurements of ¹³ C-¹³ C J-couplings are of interest. The selection of particular ¹³ C isotopomers of a glycan depends on the type of question under scrutiny. A chemical synthesis of a mannose-containing hexasaccharide,

SUBMITTER: Zhang W 

PROVIDER: S-EPMC5177528 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature