Absolute Quantitation of Human Milk Oligosaccharides Reveals Phenotypic Variations during Lactation.

ABSTRACT:

Background

The quantitation of human milk oligosaccharides (HMOs) is challenging because of the structural complexity and lack of standards.

Objective

The objective of our study was to rapidly measure the absolute concentrations of HMOs in milk using LC-mass spectrometry (MS) and to determine the phenotypic secretor status of the mothers.

Methods

This quantitative method for measuring HMO concentration was developed by using ultraperformance LC multiple reaction monitoring MS. It was validated and applied to milk samples from Malawi (88 individuals; 88 samples from postnatal month 6) and the United States (Davis, California; 45 individuals, mean age: 32 y; 103 samples collected on postnatal days 10, 26, 71, or 120, repeated measures included). The concentrations of α(

SUBMITTER: Xu G 

PROVIDER: S-EPMC5177733 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature