A20 Curtails Primary but Augments Secondary CD8⁺ T Cell Responses in Intracellular Bacterial Infection.

ABSTRACT: The ubiquitin-modifying enzyme A20, an important negative feedback regulator of NF-κB, impairs the expansion of tumor-specific CD8⁺ T cells but augments the proliferation of autoimmune CD4⁺ T cells. To study the T cell-specific function of A20 in bacterial infection, we infected T cell-specific A20 knockout (CD4-Cre A20^fl/fl) and control mice with Listeria monocytogenes. A20-deficient pathogen-specific CD8⁺ T cells expanded stronger resulting in improved pathogen control at day 7 p.i. Imaging flow cytometry revealed that A20-deficient Listeria-specific CD8⁺ T cells underwent increased apoptosis and necroptosis resulting in reduced numbers of memory CD8⁺ T cells. In contrast, the primary CD4⁺ T cell response was A2

SUBMITTER: Just S 

PROVIDER: S-EPMC5177869 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature