A simple and rapid method for optical visualization and quantification of bacteria on textiles.

ABSTRACT: To prevent bacterial contamination on textiles and the associated undesired effects different biocidal coatings have been investigated and applied. However, due to health and environmental concerns anti-adhesive coatings preventing the binding of bacteria would be favored. To develop such anti-adhesive coatings simple assays for reliable and fast screening are beneficial. Here an easy-to-handle, robust and rapid assay to assess bacteria on textiles utilizing a tetrazolium salt was reported. The assay allowed direct eye visualization of the color change of the textiles containing bacteria, facilitating fast screening. Quantification of the adhered bacteria could be done by generating standard curves which correlate the staining intensity to cell numbers. An additional advantage of the descr

SUBMITTER: Stiefel P 

PROVIDER: S-EPMC5177907 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature